The Minnesota Transportation Museum presents the history of human travel. The museum consists of three sites. The Jackson Street Roundhouse, once a maintenance building for steam engines, presents the railway histories of St. Paul, Minneapolis, and the greater Upper Midwest. This site also serves as the home of the museum's classic busses. The Osceola and St. Croix Valley Railway in Osceola, Wisconsin provides the chance to ride a train. The museum also operates Minneapolis' historic Minnehaha or "Princess" Depot.

The museum offers interactive exhibits; train, caboose, and bus rides; and the opportunity to watch restoration work in progress.