The Museum of Science and History involves the public in the exploration of local history and the natural and physical sciences.

The museum offers exhibits, planetariums shows, science demonstrations, programs and self-guided tours for school groups, programs for homeschoolers, camps, and other recreational and educational events.

Built in 1907, the Gamble Place was a winter retreat for James N. Gamble, of Procter and Gamble.

The house offers self-guided and guided tours.

The At-Tah-Thi-Ki Museum interprets the history and culture of the Florida Seminoles.

The museum offers a short film, exhibits, nature trails, craft demonstrations, guided tours for school groups, research library access,

The South Florida Museum presents and preserves local natural and cultural history.

The museum offers exhibits; film screenings; planetarium shows; manatee presentations; programs and guided and self-guided tours for school groups; in-class outreach presentations; programs for homeschoolers; professional development for educators; summer camps; and other recreational and educational events.

The Historic Arkansas Museum preserves five restored antebellum homes, where visitors can learn from living history interpreters about life in frontier Arkansas. In addition, the museum center displays art objects and artifacts from Arkansas history.

The museum offers exhibits, living history demonstrations, tours and educational programs for school groups, loan boxes, and professional development opportunities for educators.

The St. Clement's Island Museum, located at the site of Maryland's first colonial landing, guides visitors through the events that led up to that moment in 1634 and examines the industries that sprang up in Maryland as it was colonized.

The museum offers exhibits and guided tours for school groups.

The Virginia War Museum follows the history of the U.S.'s involvement in war, from the Revolutionary War to the present day, with galleries focusing on the role of women and African Americans in military history, the place of Virginia in war efforts, and the development of weaponry over the centuries.

The museum offers exhibits, educational programs and tours for school groups, in-class outreach presentations, research library access by appointment, and occasional recreational and educational events.

The U.S. Army Medical Department Museum displays equipment, uniforms, vehicles, exhibits, photos, and artwork relating to the history of the U.S. Army Medical Department from 1775 to the present.

The museum offers exhibits, research library access, and occasional recreational and educational events.

The Country Music Hall of Fame and Museum presents the history of country music through its permanent exhibit, "Sing Me Back Home," showcasing both the music and the artifacts behind and surrounding it—costumes, lyrics sheets, musical instruments. It also hosts traveling and changing exhibits and programs and performances.

The museum offers exhibits, tours and in-museum workshops for school groups, curriculum guides, and educational and recreational events.

The B. Carroll Reece Memorial Museum, part of the East Tennessee State University, houses the University's Art Department's permanent collection of works of art and a historical collection which serves to preserve the story of the settlement of Johnson City, Washington County, and the surrounding region.

The museum offers exhibits and occasional recreational and educational events, including art classes for students.