The De Soto National Memorial commemorates Spaniard Hernando de Soto's (circa 1496-1542) 1539 arrival at Tampa Bay. Following the landing, de Soto and his men encountered, and in some cases fought, local Native American groups over the course of four years. Collections include historic arms and armor. Between December and April, the site offers re-enactments and demonstrations at Camp Uzita.

The park offers a 22-minute introductory film, exhibits, helmets and armor for guests to try on, guided walking tours, nature trails with interpretive signage and wayside exhibits, 40-minute outreach presentations, Junior Ranger activities, Junior Ranger camps, and a picnic area. The website offers a suggested reading list.