The USS Midway is the longest serving 20th-century U.S. Navy aircraft carrier. Collections include 25 restored aircraft, dating from World War II through Operation Desert Storm. The majority of exhibits display the various functions of the rooms and sections aboard the Midway.

The museum offers traditional and interactive exhibits; films; three flight simulators; music videos; hands-on aircraft; two audio tours—one of which is designed for students; guided tours; a one-hour narrated video tour; docents available to answer questions; youth overnight programs; and educational programs which meet state educational standards. Reservations are required for guided tours. The site is approximately 60 percent wheelchair accessible, and wheelchairs are available upon request. Student audio tours are available in English and Spanish.